The T2T-NIP provides complete sequences of a gapless telomere-to-telomere assembly, T2T-NIP, for the rice reference genome (Oryza sativa ssp. Japonica cv. Nipponbare) and functional element annotation of the 12 complete rice chromosomes. Large improvements have been made in this T2T-NIP which resolves the remaining 3% unknown genomic regions in IRGSP-1.0. These data are available through search pages and our Genome Browser that provides an integrated display of annotation data.